Uncovering cargo clients and accessory factors of AP-1 and AP-4 through vesicle proteomics

Proceedings of the National Academy of Sciences, Volume 122, Number 40, October 2025.
ImportanceThe adapter protein complexes AP-1 and AP-4 mediate protein sorting within the trans-Golgi network (TGN), but their cargo clients and regulators remain incompletely defined. Here, using a large-scale vesicle formation assay coupled with a label-free assay…