NEXT-FRET maps nonequilibrium rerouting of Escherichia coli maltose-binding protein folding by its signal peptide and chaperones

Proceedings of the National Academy of Sciences, Volume 123, Number 20, May 2026.
ImportanceProteins and other biomolecules function through non-equilibrium transient intermediates that are crucial for function. Even folding, by which proteins achieve native forms despite the astronomical possibilities posed by Levinthal’s paradox,…