Phage-based delivery of CRISPR-associated transposases for targeted bacterial editing

Proceedings of the National Academy of Sciences, Volume 122, number 30, July 2025.
Significant microbial communities live and have an impact on most ecosystems on planet Earth. Although CRISPR -based technologies have enabled a flexible modification of the bacterial genome and in vitro phage in the last decade, there is a need for new delivery …