Ludwig–Soret microscopy with the vibrational photothermal effect

Proceedings of the National Academy of Sciences, Volume 122, Issue 33, August 2025. <br/>SignificanceWe introduce vibrational photothermal-induced Soret (ViPS) imaging, an approach extending vibrational microscopy into the study of intracellular thermophoresis—the transport of biomolecules driven by temperature gradients. By combining steady-…