A chemical epigenetic tool to probe site-specific DNA-binding protein complexes

Proceedings of the National Academy of Sciences, Volume 122, Number 40, October 2025.
Significance We incorporated a photo-cross-linking non-canonical amino acid into a catalytically deficient CRISPR/Cas9 in human pluripotent stem cells (hPSCs) and their derived cell types. Guided by specially designed gRNAs, this modified protein could…