A FRET assay to monitor different structural states of human β-cardiac myosin including the interacting-heads motif

Proceedings of the National Academy of Sciences, Volume 122, Issue 34, August 2025. <br/>SignificanceThe stability of the folded back blocked head configuration (BHC) of the interacting-heads motif (IHM) state of β-cardiac myosin is linked to the pathological effects of myosin cardiomyopathy mutations. Myosin sequestration is often measured …