In vitro maturation of fully active [FeFe]-hydrogenase in a defined system including the iron carrier NfuA

Proceedings of the National Academy of Sciences, Volume 122, Issue 39, September 2025. <br/>Significance[FeFe]-hydrogenases catalyze the reduction of protons to produce the clean fuel H2, and thus have important potential in green energy applications. Harnessing the power of hydrogenases requires efficient assembly of the [6Fe] catalytic cluster …