Neutron crystallography of the covalent intermediate of β-glucosidase reveals remodeling of the catalytic center

Proceedings of the National Academy of Sciences, Volume 122, Issue 44, November 2025. <br/>SignificanceGlycoside hydrolases (GHs) perform diverse functions in organisms, with over 180 known enzyme families. Although hydrogen bonds and proton transfer play important roles in enzymatic reactions, hydrogen atoms are generally invisible in …