Profiling translated regions in viral genomes at scale

Although sequencing the viral genome has progressed rapidly, the functional annotation of elements such as the translated regions is always incomplete for most viruses. Calculation approaches are faced with challenges in the profiling of open reading frames (ORF) and experimental methods are limited by a low speed. To write ScienceWeingarten-Gabbay et al. Now report a massively parallel ribosome profiling method to detect regions translated into hundreds of viruses in a single experience. The authors transferred a synthetic oligonucleotide library – in which each oligonucleotide contained a viral sequence of 200 nucleotides flanked by constant primers and cloned in an overexpression vector – in two human cell lines and then made a ribosome profiling. The oligonucleotides lasted the unrevived region 5 ‘and the start of the coding sequence of 3,976 genes in 679 viral genomes.

The profiling of ribosomes identified 4,208 non -canonical orf. When the authors compared the scheme of ribosomes in the synthetic library with four native viral infections, they found a strong alignment between the location of the imprints from each analysis.