Reaching the full potential of cryo-EM reconstructions with molecular dynamics simulations at 310 K: Actin filaments as an example

Proceedings of the National Academy of Sciences, Volume 122, Number 48, December 2025.
ImportanceThe functionally important properties of proteins are closely related to their conformations at physiological temperatures. While cryo-electron microscopy (cryo-EM) reconstructions and crystal structure of frozen proteins provide …