Scalable secondary metabolite production in Streptomyces using a plug-and-play system

https://www.profitableratecpm.com/f4ffsdxe?key=39b1ebce72f3758345b2155c98e6709c
  • Chater, K. F. Streptomyces inside-out: a new perspective on the bacteria that provide us with antibiotics. Philos. Trans. R. Soc. Lond. B Biol. Sci. 361, 761–768 (2006).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Hutchings, M. I., Truman, A. W. & Wilkinson, B. Antibiotics: past, present and future. Curr. Opin. Microbiol. 51, 72–80 (2019).

    PubMed 

    Google Scholar 

  • Zhang, L. & Demain, A. L. (eds) Natural Products: Drug Discovery and Therapeutic Medicine. (Humana Press, 2005).

  • Li, S. et al. Polyketide pesticides from actinomycetes. Curr. Opin. Biotechnol. 69, 299–307 (2021).

    PubMed 

    Google Scholar 

  • Wang, W. et al. Harnessing the intracellular triacylglycerols for titer improvement of polyketides in Streptomyces. Nat. Biotechnol. 38, 76–83 (2020).

    PubMed 

    Google Scholar 

  • Yan, H., Li, S. & Wang, W. Reprogramming naturally evolved switches for Streptomyces chassis development. Trends Biotechnol. 43, 12–15 (2025).

    PubMed 

    Google Scholar 

  • Zhang, Y. X. et al. Genome shuffling leads to rapid phenotypic improvement in bacteria. Nature 415, 644–646 (2002).

    PubMed 

    Google Scholar 

  • Li, J. et al. A non-carboxylative route for the efficient synthesis of central metabolite malonyl-CoA and its derived products. Nat. Catal. 7, 361–374 (2024).

    Google Scholar 

  • Thaker, M. N. et al. Identifying producers of antibacterial compounds by screening for antibiotic resistance. Nat. Biotechnol. 31, 922–927 (2013).

    PubMed 

    Google Scholar 

  • Barka, E. A. et al. Taxonomy, physiology, and natural products of actinobacteria. Microbiol. Mol. Biol. Rev. 80, 1–43 (2016).

    PubMed 

    Google Scholar 

  • Nett, M., Ikeda, H. & Moore, B. S. Genomic basis for natural product biosynthetic diversity in the actinomycetes. Nat. Prod. Rep. 26, 1362–1384 (2009).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Miethke, M. et al. Towards the sustainable discovery and development of new antibiotics. Nat. Rev. Chem. 5, 726–749 (2021).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Montano Lopez, J., Duran, L. & Avalos, J. L. Physiological limitations and opportunities in microbial metabolic engineering. Nat. Rev. Microbiol. 20, 35–48 (2022).

    PubMed 

    Google Scholar 

  • Wang, W. et al. Angucyclines as signals modulate the behaviors of Streptomyces coelicolor. Proc. Natl Acad. Sci. USA 111, 5688–5693 (2014).

    PubMed 
    PubMed Central 

    Google Scholar 

  • van Wezel, G. P. & McDowall, K. J. The regulation of the secondary metabolism of Streptomyces: new links and experimental advances. Nat. Prod. Rep. 28, 1311–1333 (2011).

    PubMed 

    Google Scholar 

  • Li, S., Li, Z., Pang, S., Xiang, W. & Wang, W. Coordinating precursor supply for pharmaceutical polyketide production in Streptomyces. Curr. Opin. Biotechnol. 69, 26–34 (2021).

    PubMed 

    Google Scholar 

  • Qiu, S. et al. Building a highly efficient Streptomyces super-chassis for secondary metabolite production by reprogramming naturally-evolved multifaceted shifts. Metab. Eng. 81, 210–226 (2023).

    PubMed 

    Google Scholar 

  • Wu, J. et al. Developing a pathway-independent and full-autonomous global resource allocation strategy to dynamically switching phenotypic states. Nat. Commun. 11, 5521 (2020).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Gupta, A., Reizman, I. M., Reisch, C. R. & Prather, K. L. Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit. Nat. Biotechnol. 35, 273–279 (2017).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Din, M. O. et al. Synchronized cycles of bacterial lysis for in vivo delivery. Nature 536, 81–85 (2016).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Alnahhas, R. N. et al. Majority sensing in synthetic microbial consortia. Nat. Commun. 11, 3659 (2020).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Polkade, A. V., Mantri, S. S., Patwekar, U. J. & Jangid, K. Quorum sensing: an under-explored phenomenon in the phylum actinobacteria. Front. Microbiol. 7, 131 (2016).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Biarnes-Carrera, M., Breitling, R. & Takano, E. Butyrolactone signalling circuits for synthetic biology. Curr. Opin. Chem. Biol. 28, 91–98 (2015).

    PubMed 

    Google Scholar 

  • Zhou, S. et al. Molecular basis for control of antibiotic production by a bacterial hormone. Nature 590, 463–467 (2021).

    PubMed 

    Google Scholar 

  • Takano, E., Chakraburtty, R., Nihira, T., Yamada, Y. & Bibb, M. J. A complex role for the gamma-butyrolactone SCB1 in regulating antibiotic production in Streptomyces coelicolor A3(2). Mol. Microbiol. 41, 1015–1028 (2001).

    PubMed 

    Google Scholar 

  • Corre, C., Song, L., O’Rourke, S., Chater, K. F. & Challis, G. L. 2-Alkyl-4-hydroxymethylfuran-3-carboxylic acids, antibiotic production inducers discovered by Streptomyces coelicolor genome mining. Proc. Natl Acad. Sci. USA 105, 17510–17515 (2008).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Kitani, S. et al. Avenolide, a Streptomyces hormone controlling antibiotic production in Streptomyces avermitilis. Proc. Natl Acad. Sci. USA 108, 16410–16415 (2011).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Wang, W. et al. Identification of a butenolide signaling system that regulates nikkomycin biosynthesis in Streptomyces. J. Biol. Chem. 293, 20029–20040 (2018).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Cuthbertson, L. & Nodwell, J. R. The TetR family of regulators. Microbiol. Mol. Biol. Rev. 77, 440–475 (2013).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Wang, W. et al. An engineered strong promoter for Streptomycetes. Appl. Environ. Microbiol. 79, 4484–4492 (2013).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Bhukya, H., Bhujbalrao, R., Bitra, A. & Anand, R. Structural and functional basis of transcriptional regulation by TetR family protein CprB from S. coelicolor A3(2). Nucleic Acids Res. 42, 10122–10133 (2014).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Wang, J. et al. A novel role of ‘pseudo’γ-butyrolactone receptors in controlling γ-butyrolactone biosynthesis in Streptomyces. Mol. Microbiol. 82, 236–250 (2011).

    PubMed 

    Google Scholar 

  • Horbal, L., Fedorenko, V. & Luzhetskyy, A. Novel and tightly regulated resorcinol and cumate-inducible expression systems for Streptomyces and other actinobacteria. Appl. Microbiol. Biotechnol. 98, 8641–8655 (2014).

    PubMed 

    Google Scholar 

  • Wang, X., Fu, Y., Wang, M. & Niu, G. Synthetic cellobiose-inducible regulatory systems allow tight and dynamic controls of gene expression in Streptomyces. ACS Synth. Biol. 10, 1956–1965 (2021).

    PubMed 

    Google Scholar 

  • Hou, J. et al. Engineering the ultrasensitive transcription factors by fusing a modular oligomerization domain. ACS Synth. Biol. 7, 1188–1194 (2018).

    PubMed 

    Google Scholar 

  • Lou, C. et al. Synthesizing a novel genetic sequential logic circuit: a push‐on push‐off switch. Mol. Syst. Biol. 6, 350 (2010).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Mascher, T. Past, present, and future of extracytoplasmic function σ factors: distribution and regulatory diversity of the third pillar of bacterial signal transduction. Annu. Rev. Microbiol. 77, 625–644 (2023).

    PubMed 

    Google Scholar 

  • Seipke, R. F., Patrick, E. & Hutchings, M. I. Regulation of antimycin biosynthesis by the orphan ECF RNA polymerase sigma factor σAntA. PeerJ 2, e253 (2014).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Bai, C. et al. Exploiting a precise design of universal synthetic modular regulatory elements to unlock the microbial natural products in Streptomyces. Proc. Natl Acad. Sci. USA 112, 12181–12186 (2015).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Sun, P. et al. Spiroketal formation and modification in avermectin biosynthesis involves a dual activity of AveC. J. Am. Chem. Soc. 135, 1540–1548 (2013).

    PubMed 

    Google Scholar 

  • Kitani, S., Ikeda, H., Sakamoto, T., Noguchi, S. & Nihira, T. Characterization of a regulatory gene, aveR, for the biosynthesis of avermectin in Streptomyces avermitilis. Appl. Microbiol. Biotechnol. 82, 1089–1096 (2009).

    PubMed 

    Google Scholar 

  • Qiu, J. et al. Overexpression of the ABC transporter AvtAB increases avermectin production in Streptomyces avermitilis. Appl. Microbiol. Biotechnol. 92, 337–345 (2011).

    PubMed 

    Google Scholar 

  • Hao, Y. et al. Avermectin B1a production in Streptomyces avermitilis is enhanced by engineering aveC and precursor supply genes. Appl. Microbiol. Biotechnol. 106, 2191–2205 (2022).

    PubMed 

    Google Scholar 

  • Yang, M., Hao, Y., Liu, G. & Wen, Y. Enhancement of acyl-CoA precursor supply for increased avermectin B1a production by engineering meilingmycin polyketide synthase and key primary metabolic pathway genes. Microb. Biotechnol. 17, e14470 (2024).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Madduri, K. et al. Production of the antitumor drug epirubicin (4′-epidoxorubicin) and its precursor by a genetically engineered strain of Streptomyces peucetius. Nat. Biotechnol. 16, 69–74 (1998).

    PubMed 

    Google Scholar 

  • Malla, S., Niraula, N. P., Liou, K. & Sohng, J. K. Improvement in doxorubicin productivity by overexpression of regulatory genes in Streptomyces peucetius. Res. Microbiol. 161, 109–117 (2010).

    PubMed 

    Google Scholar 

  • Malla, S., Niraula, N. P., Liou, K. & Sohng, J. K. Enhancement of doxorubicin production by expression of structural sugar biosynthesis and glycosyltransferase genes in Streptomyces peucetius. J. Biosci. Bioeng. 108, 92–98 (2009).

    PubMed 

    Google Scholar 

  • Scotti, C. & Hutchinson, C. R. Enhanced antibiotic production by manipulation of the Streptomyces peucetius dnrH and dnmT genes involved in doxorubicin (adriamycin) biosynthesis. J. Bacteriol. 178, 7316–7321 (1996).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Song, E. et al. Proteomic approach to enhance doxorubicin production in panK-integrated Streptomyces peucetius ATCC 27952. J. Ind. Microbiol. Biotechnol. 38, 1245–1253 (2011).

    PubMed 

    Google Scholar 

  • Ryu, Y. G., Butler, M. J., Chater, K. F. & Lee, K. J. Engineering of primary carbohydrate metabolism for increased production of actinorhodin in Streptomyces coelicolor. Appl. Environ. Microbiol. 72, 7132–7139 (2006).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Alam, K. et al. Streptomyces: the biofactory of secondary metabolites. Front. Microbiol. 13, 968053 (2022).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Breitling, R. et al. Synthetic biology approaches to actinomycete strain improvement. FEMS Microbiol. Lett. 368, fnab060 (2021).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Breitling, R. & Takano, E. Synthetic biology of natural products. Cold Spring Harb. Perspect. Biol. 8, a023994 (2016).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Moser, F. et al. Genetic circuit performance under conditions relevant for industrial bioreactors. ACS Synth. Biol. 1, 555–564 (2012).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Trosset, J. Y. & Carbonell, P. Synergistic synthetic biology: units in concert. Front. Bioeng. Biotechnol. 1, 11 (2013).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Xia, P. F., Ling, H., Foo, J. L. & Chang, M. W. Synthetic genetic circuits for programmable biological functionalities. Biotechnol. Adv. 37, 107393 (2019).

    PubMed 

    Google Scholar 

  • Kieser, T., Bibb, M. J., Buttner, M. J., Chater, K. F. & Hopwood, D. A. Practical Streptomyces Genetics (The John Innes Foundation, 2000).

  • Wang, W. X. et al. Identification of a butenolide signaling system that regulates nikkomycin biosynthesis in Streptomyces. J. Biol. Chem. 293, 20029–20040 (2018).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Rodriguez-Garcia, A., Combes, P., Perez-Redondo, R., Smith, M. C. & Smith, M. C.Natural and synthetic tetracycline-inducible promoters for use in the antibiotic-producing bacteria Streptomyces. Nucleic. Acids Res. 33, e87 (2005).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Wang, W. et al. Development of a synthetic oxytetracycline-inducible expression system for Streptomycetes using de novo characterized genetic parts. ACS Synth. Biol. 5, 765–773 (2016).

    PubMed 

    Google Scholar 

  • Blin, K., Pedersen, L. E., Weber, T. & Lee, S. Y. CRISPy-web: an online resource to design sgRNAs for CRISPR applications. Synth. Syst. Biotechnol. 1, 118–121 (2016).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Yan, H. et al. A rational multi-target combination strategy for synergistic improvement of non-ribosomal peptide production. Nat. Commun. 16, 1883 (2025).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Chen, H. H. et al. High-yield porphyrin production through metabolic engineering and biocatalysis. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02267-3 (2024).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Cao, J. et al. Harnessing a previously unidentified capability of bacterial allosteric transcription factors for sensing diverse small molecules in vitro. Sci. Adv. 4, eaau4602 (2018).

    PubMed 
    PubMed Central 

    Google Scholar 

  • Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods 25, 402–408 (2001).

    PubMed 

    Google Scholar 

  • Zhou, H. et al. Systematic development of a highly efficient cell factory for 5-aminolevulinic acid production. Trends Biotechnol. 42, 1479–1502 (2024).

    PubMed 

    Google Scholar 

  • Related Articles

    Leave a Reply

    Your email address will not be published. Required fields are marked *

    Back to top button