Synthesis of large single-transcript pathways from oligonucleotide pools: Design of STARBURST, an autobioluminescent reporter

Proceedings of the National Academy of Sciences, Volume 122, Issue 31, August 2025. <br/>SignificanceLarge-scale DNA synthesis from oligonucleotide pools is valuable for synthetic biology as it enables low-cost gene synthesis. Here, we developed a simple computational–experimental pipeline,iggypop, that enables rapid gene synthesis and …