Heterogeneous template-dependent transcription dynamics of T7 RNAP revealed by single-molecule imaging
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Proceedings of the National Academy of Sciences, Volume 122, Number 50, December 2025.
Importance Bacteriophage T7 RNA polymerase is a key tool in RNA synthesis, but its efficiency can be hampered by premature stalls and low yields. This study highlights the causes of these limitations: the flexibility and electrostatic potential of …
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