Optimization of a sarbecovirus llama nanobody–antigen binding interface via a combined computational and phage display protein engineering approach

Proceedings of the National Academy of Sciences, Volume 122, number 28, July 2025.
significant and the power of nanobodies can be improved using mutations designed by calculation. The display of phages provides an effective way to explore random up to 1010 unique muteines simultaneously. However, a typical antibody-antigen interface …