Microscopic and structural observations of actin filament capping and severing by cytochalasin D

Proceedings of the National Academy of Sciences, Volume 122, Issue 29, July 2025. <br/>SignificanceThe regulatory mechanism of cytochalasin D (CytoD), a widely used actin inhibitor, was elucidated through real-time microscopy and X-ray crystallography. Nanomolar concentrations of CytoD completely halt polymerization and depolymerization at …