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Proceedings of the National Academy of Sciences, Volume 122, Issue 33, August 2025. <br/>SignificanceF1-ATPase is a rotary molecular motor that forms part of the ATP synthase complex. The F1-ATPase from thermophilicBacillusPS3 rotates in 120° steps upon ATP hydrolysis, consisting of two substeps of 80° and 40°. In this study, we analyzed …
