Neutron and time-resolved X-ray crystallography reveal the substrate recognition and catalytic mechanism of human Nudix hydrolase MTH1

Proceedings of the National Academy of Sciences, Volume 122, number 29, July 2025.
Importance to the state of protonment / deprotonation at the active enzymatic site is crucial for catalytic reactions. However, states of protonment / deprottonation of amino acid residues have rarely been demonstrated experimentally due to the challenges of …