In situ NMR reveals a pH sensor motif in an outer membrane protein that drives bacterial vesicle production

Proceedings of the National Academy of Sciences, Volume 122, Issue 26, July 2025. <br/>SignificanceTheSalmonellasurface protein PagC is a major driver of extracellular vesicle formation. Here, we show that this phenotype is transferrable toEscherichia coli, another diderm, Gram-negative bacterium. PagC increases vesicle production inE. …