Phage display–mediated immuno-PCR to detect low-abundance secreted proteins in Drosophila

Proceedings of the National Academy of Sciences, Volume 123, Number 15, April 2026.
ImportanceHormones and other secreted factors orchestrate body-wide physiology, but their routine quantification in Drosophila has been limited by the limited volume of hemolymph available for tests such as the enzyme-linked immunosorbent assay. Here we…